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Motion Color drop in water, Ink swirling in, Colorful ink abstraction. In paper based microfluidics, capillary elements can be achieved through the simple variation of section geometry. Continuous-flow microfluidics Ĭontinuous flow microfluidics rely on the control of a steady state liquid flow through narrow channels or porous media predominantly by accelerating or hindering fluid flow in capillary elements. Disadvantages to open systems include susceptibility to evaporation, contamination, and limited flow rate. Examples of open microfluidics include open-channel microfluidics, rail-based microfluidics, paper-based, and thread-based microfluidics. In addition, open microfluidics eliminates the need to glue or bond a cover for devices, which could be detrimental to capillary flows. Open microfluidic devices are also easy and inexpensive to fabricate by milling, thermoforming, and hot embossing. Another advantage of open microfluidics is the ability to integrate open systems with surface-tension driven fluid flow, which eliminates the need for external pumping methods such as peristaltic or syringe pumps. Advantages of open microfluidics include accessibility to the flowing liquid for intervention, larger liquid-gas surface area, and minimized bubble formation. In open microfluidics, at least one boundary of the system is removed, exposing the fluid to air or another interface (i.e. The behavior of fluids and their control in open microchannels was pioneered around 2005 and applied in air-to-liquid sample collection and chromatography. Each of these methods has its own associated techniques to maintain robust fluid flow which have matured over several years. Traditionally, microfluidic flows have been generated inside closed channels with the channel cross section being in the order of 10 μm x 10 μm. Microfluidic flows need only be constrained by geometrical length scale - the modalities and methods used to achieve such a geometrical constraint are highly dependent on the targeted application. High specificity of chemical and physical properties (concentration, pH, temperature, shear force, etc.) can also be ensured resulting in more uniform reaction conditions and higher grade products in single and multi-step reactions. A key consequence is co-flowing fluids do not necessarily mix in the traditional sense, as flow becomes laminar rather than turbulent molecular transport between them must often be through diffusion. In particular, the Reynolds number (which compares the effect of the momentum of a fluid to the effect of viscosity) can become very low. Īt small scales (channel size of around 100 nanometers to 500 micrometers) some interesting and sometimes unintuitive properties appear. Microfluidics studies how these behaviours change, and how they can be worked around, or exploited for new uses. The behaviour of fluids at the microscale can differ from "macrofluidic" behaviour in that factors such as surface tension, energy dissipation, and fluidic resistance start to dominate the system. Bottom: Phase contrast micrographs of a serpentine channel ~15 μm wide. Silicone rubber and glass microfluidic devices. Often, processes normally carried out in a lab are miniaturised on a single chip, which enhances efficiency and mobility, and reduces sample and reagent volumes. Microvalves determine the flow direction or the mode of movement of pumped liquids. Micropumps supply fluids in a continuous manner or are used for dosing. Active microfluidics refers to the defined manipulation of the working fluid by active (micro) components such as micropumps or microvalves. Examples are rotary drives applying centrifugal forces for the fluid transport on the passive chips. In some applications, external actuation means are additionally used for a directed transport of the media. Various applications rely on passive fluid control using capillary forces, in the form of capillary flow modifying elements, akin to flow resistors and flow accelerators. Typically microfluidic systems transport, mix, separate, or otherwise process fluids. Typically, micro means one of the following features: Microfluidics emerged in the beginning of the 1980s and is used in the development of inkjet printheads, DNA chips, lab-on-a-chip technology, micro-propulsion, and micro-thermal technologies. It has practical applications in the design of systems that process low volumes of fluids to achieve multiplexing, automation, and high-throughput screening. It is a multidisciplinary field that involves molecular analysis, biodefence, molecular biology, and microelectronics. Microfluidics refers to a system that manipulates a small amount of fluids ((10 -9 to 10 -18 liters) using small channels with sizes ten to hundreds micrometres. Accordingly, a definitive plan can be created for managing total wealth while one is alive with a clear plan to pass on the legacy. It is an opportunity to contemplate and define what wealth really means to a family. Legacy planning helps you to establish goals for the ultimate disposition of your wealth. This makes estate and succession planning extremely crucial for creating a successful legacy. Researchers have suggested that there is a dilution of wealth across generations, about 70% by the second generation and 90% by the third, and is a challenge almost all wealthy families face. Whenever wealth and leadership is transferred to the next generation, there is an air of uncertainty of what the future will bring. 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It creates an opportunity that many non-federal fire departments already enjoy successfully – with no appreciable cost to the employer.” Because safety is so important to firefighters and EMS providers, they must function on the job unimpaired and fully focused. Schaitberger says, “This important legislation gives federal fire fighters the ability to better manage their challenging schedules to create better work-life balances. The House companion bill, HR 2059, was introduced simultaneously by Representative John Sarbanes (D-MD). He is joined by original cosponsors Senators Tom Carper (D-DE), Susan Collins (R-ME) and Jon Tester (D-MT). The Senate bill, S 838, is being led by Senator Chris Van Hollen (D-MD). The Federal Firefighters Flexibility and Fairness Act allowing federal fire fighters to swap shifts has been reintroduced by a bicameral and bipartisan group of legislators. 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Seven Spanish Angels is a song written by Troy Seals and Eddie Setser, and recorded by Ray Charles as a duet with Willie Nelson. This is followed by "thunder from the throne" and the angels "tak another angel home". After each death, the titular angels gather to pray for the lovers. She tearfully prays, "Father, please forgive me I can't make it without my man." She deliberately points the empty weapon at the lawmen and is then shot dead. He is immediately shot and killed, prompting his distraught lover to pick up his rifle. The gunfight then commences, with the outlaw firing upon the posse. In 1 Enoch 20, the Greek text has seven angels: Uriel, Raphael, Raguel, Michael, Sariel, Gabriel, and Remeiel (missing in the Ethiopic text, see OTP 1:2324). In Testament of Levi 8, Levi sees seven men clothed in white who prepare him to be a priest. Before the final gunfight, the two embrace, speaking of their belief that God will spare them. The tradition of seven archangels is present in the apocryphal book of Tobit. When they are cornered, they decide to fight the approaching lawmen. The song is about an outlaw and his lover who are trying to outrun a posse sent to return them to Texas. Sherrill proposed that Nelson and Charles duet on the song after learning of Nelson's having reserved it. Before Nelson was able to record it, producer Billy Sherrill happened to hear the demo and wanted the song for Ray Charles. " Within two days "Seven Spanish Angels" had been successfully pitched to Willie Nelson. Setser had suggested the title "Seven Spanish Angels" and he and Seals had written the song as a homage to the tejano flavored classic hits of Marty Robbins exemplified by Robbins' career record " El Paso" (Troy Seals quote): "When we finished it we thought 'Who in the world's gonna do it?' because Marty was. The single spent one week at number one and a total of twelve weeks on the country chart. "Seven Spanish Angels" was the most successful of Charles' eight hits on the country chart. It was also included on Nelson's 1985 compilation album Half Nelson. Charles and Nelson split the verses, with Charles singing the first and Nelson the second, Charles sang the first and second choruses with Nelson joining for the outro. It was released in November 1984 as a single from Charles' 1984 album Friendship. " Seven Spanish Angels" is a song written by Troy Seals and Eddie Setser, and recorded by Ray Charles as a duet with Willie Nelson. A7 She said, 'Father, please forgive me, D I can't. Verse D She reached down and picked the gun up, A That lay smoking in his hand. 1984 single by Ray Charles with Willie Nelson "Seven Spanish Angels" Provided to YouTube by IIP-DDSSeven Spanish Angels Willie Nelson and Ray CharlesCountry's Greatest Hits PMIReleased on: Artist. 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